![]() The present studies were conducted to characterize expression of an inhibitor of apoptosis ( iap) gene, inhibitor of T-cell apoptosis ( ita), within hen follicle tissues at various stages of follicle development. By comparison, prehierarchal (≤ 8-mm diameter) follicles are highly susceptible to becoming atretic in vivo, and approximately 70% of granulosa cells collected from 3- to 8-mm-diameter follicles rapidly undergo apoptosis when incubated for as little as 6 h in vitro in defined medium. It is now well established that within the hen ovary, preovulatory follicles rarely become atretic and that granulosa cells from preovulatory follicles are relatively resistant to undergoing apoptosis in vitro.
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